Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain
Table 1
Polymorphisms between the current cDNAs sequenced from salivary glands of Phlebotomus perniciosus from Madrid and their corresponding best matches in NR database.
Protein name/NCBI accession number
Best match to NR nucleotide database
Protein family
BLASTn identities
Nucleotidic polymorphismsa
Aminoacidic polymorphismsa
Changes in glycosylation sitesc
Changes in phosphorylation sitesd
Changes in protein structuree
Gaps
Identities
UTRs
CDS
Antigenic regionsb
Nonantigenic regionsb
N
O
SP01 (HE974344)
PperSP01 (DQ192490)
Apyrase
0/999 (0%)
982/999 (98%)
0
17
1
1
No
No
Yes
No
SP01B (HE974345)
PperSP01B (DQ192491)
Apyrase
16/1116 (1%)
1089/1116 (98%)
3
8
4
2
No
No
Yes
Yes
SP02 (HE985074)
PperSP02 (DQ150620)
PpSP15-like
0/522 (0%)
513/522 (98%)
3
6
2
1
No
No
Yes
No
SP02 (HE985075)
PperSP02 (DQ150620)
PpSP15-like
1/523 (0%)
513/523 (98%)
3
6
3f
0f
No
No
Yes
Yes
SP02 (HE985076)
PperSP02 (DQ150620)
PpSP15-like
2/524 (0%)
511/524 (98%)
2
9
5
1
No
No
Yes
No
SP02 (HE985077)
PperSP02 (DQ150620)
PpSP15-like
3/525 (0%)
511/525 (97%)
1
10
4
1
No
No
Yes
No
SP03B (HE974346)
PperSP03B (DQ150622)
Yellow protein
0/1182 (0%)
1165/1182 (99%)
0
17
4
4
No
No
Yes
Yes
SP04 (HE980444)
PperSP04 (DQ150623)
D7-related
0/838 (0%)
821/838 (98%)
5
12
4
1
No
No
No
No
SP04B (HE980443)
PperSP04B (DQ150624)
D7-related
2/824 (0%)
761/824 (92%)
9
52
22
5
No
No
Yes
Yes
SP06 (HE970770)
PperSP06 (DQ153100)
Lufaxin
1/1031 (0%)
1011/1031 (98%)
8
11
3
1
No
No
Yes
Yes
SP08 (HE974347)
PperSP08 (DQ153102)
ParSP25-like
0/774 (0%)
766/774 (99%)
0
8
3
1
No
No
Yes
Yes
SP09 (HE966456)
PperSP09 (DQ153103)
PpSP15-like
0/500 (0%)
477/500 (95%)
7
16
8
1
No
No
Yes
Yes
SP11 (HE974348)
PperSP11 (DQ153105)
PpSP15-like
10/483 (2%)
458/483 (95%)
11
4
1
0
No
No
No
No
Hypothetical protein P119 (HE985078)
Aedes aegypti hypothetical protein (XM_001663068)
Unknown
0/496 (0%)
402/496 (81%)
18
76
8
1
No
Yes
Yes
Yes
Nucleotidic and aminoacid polymorphisms between the current sequenced cDNA clones and their best P. perniciosus salivary cDNA matches (BLASTn and ClustalW for nucleotidic and amino acidic alignments). The number of nucleotidic polymorphisms fits the values of nonidentical nucleotides (column 5) minus the gaps (column 4). Protein antigenic regions were predicted by in silico bioinformatics (Jameson-Wolf index, Protean program, DNAstar, Lasergene). N-, and O-glycosylation sites prediction performed with NetNGlyc 1.0 Server and NetOGlyc 3.1 Server. Phosphorylation sites prediction performed with NetPhos 2.0 Server. Alpha helix and beta sheet locations were predicted by Garnier-Robson algorithm (Protean program, DNASTAR, Lasergene). Two deletions in the nucleotidic sequence of SP02 (HE985075) lead to a switch of the reading frame of the gene.