Figure 1: Efflux of K+ and effects on acidification of C. albicans strain ATCC 10231 produced by variable concentrations of chitosan. In (a) and (b), the incubation medium was 2 mM MES-TEA buffer, pH 6.0, and 20 mM glucose. At the start of the tracings, 10 μM KCl was added as a concentration reference, followed by starved cells (125 mg, wet weight). After the uptake of K+ was complete, the indicated concentrations of chitosan were added. In (b), the tracings of the acidification of the medium are shown. In (c), different amounts of cells were added as indicated with a constant chitosan concentration.