Effects of Chitosan on Candida albicans: Conditions for Its Antifungal Activity
Figure 3
Effects of chitosan on the estimated electric plasma membrane potential difference on the strain C. albicans ATCC 10231. The cells (25 mg) were added to the spectrofluorometer cell containing 10 mM MES-TEA buffer, 20 μM CCCP, 10 μM BaCl2, and 20 mM glucose, in a final volume of 2.0 mL. After 30 s, 200 nM of the cyanine DiSC3(3) was added. Where indicated, 20 mM KCl or variable amounts of chitosan was added. The fluorescence changes, 540–590 nm, for excitation and emission, respectively, were followed. AU: arbitrary units.
