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BioMed Research International
Volume 2013, Article ID 538051, 8 pages
Methodology Report

Using the Developmental Gene Bicoid to Identify Species of Forensically Important Blowflies (Diptera: Calliphoridae)

1Department of Legal Medicine, College of Medicine, Korea University, 126-1 Anamdong 5-ga, Seongbuk-gu, Seoul 136-705, Republic of Korea
2Department of Anesthesiology and Pain Medicine, CHA University Medical College, 43 Beolmal 30th-road, Gyeonggi-do, Seongnam 463-836, Republic of Korea
3Beijing Scales Forensic Center, 100 Chaoyangru, Chaoyang-qu, Beijing 100025, China
4Institute of Environmental Ecology, College of Science and Technology, Korea University, 126-1 Anamdong 5-ga, Seongbuk-gu, Seoul 136-705, Republic of Korea
5Department of Science Education, Chinju National University of Education, 380 Shinandong, Gyeongsangnam-do, Jinju 660-756, Republic of Korea

Received 19 October 2012; Revised 7 January 2013; Accepted 16 January 2013

Academic Editor: Sanford I. Bernstein

Copyright © 2013 Seong Hwan Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1: The primers used for the initial and second PCR amplification.

Supplementary Table 2: The species-specific primer pairs used.

Supplementary Table 3: Inter-specific distances of the bcd coding sequences.

Supplementary Table 4: Inter-specific distances of the bcd intron 2.

Supplementary Figure 1: The bicoid genomic structures of Drosophila melanogaster, Drosophila simulans and Musca domestica.

Supplementary Figure 2: Multiple alignments of the bcd homeodomain amino acid sequences in dipteran species.

  1. Supplementary Tables
  2. Supplementary Figure 1
  3. Supplementary Figure 2