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BioMed Research International
Volume 2013, Article ID 538790, 12 pages
Review Article

Improved Succinate Production by Metabolic Engineering

1Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China
2Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

Received 27 November 2012; Revised 12 March 2013; Accepted 17 March 2013

Academic Editor: Eugénio Ferreira

Copyright © 2013 Ke-Ke Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Succinate is a promising chemical which has wide applications and can be produced by biological route. The history of the biosuccinate production shows that the joint effort of different metabolic engineering approaches brings successful results. In order to enhance the succinate production, multiple metabolical strategies have been sought. In this review, different overproducers for succinate production, including natural succinate overproducers and metabolic engineered overproducers, are examined and the metabolic engineering strategies and performances are discussed. Modification of the mechanism of substrate transportation, knocking-out genes responsible for by-products accumulation, overexpression of the genes directly involved in the pathway, and improvement of internal NADH and ATP formation are some of the strategies applied. Combination of the appropriate genes from homologous and heterologous hosts, extension of substrate, integrated production of succinate, and other high-value-added products are expected to bring a desired objective of producing succinate from renewable resources economically and efficiently.