| | | | Succinate production | | | | Strains | Engineering strategies | Culture methods | Concentration (g L−1) | Productivity (g L−1 h−1) | Yield (g g−1) | Biomass | By-product | References |
| Mannheimia succiniciproducens |
| LPK7 | Deletion of the LDH, PFL, PTA, ACK | Anaerobic, batch | 13.4 | 1.18 | 0.67 | 1.5 g L−1 | Pyruvate, malate | | | | Anaerobic, fed-batch | 52.4 | 1.75 | 0.76 | 2.5 g L−1 | Pyruvate, malate |
[15] |
| Escherichia coli |
| JCL1208 (pCP201) | Overexpressed PEPC | Anaerobic, batch | 10.7 | 0.59 | 0.29 | — | Ethanol, acetate, lactate, formate | [16] | MG1655-pyc | Overexpressed PYC | Anaerobic, batch | 1.77 | 0.177 | 0.177 | 0.9 g L−1 | Lactate, formate | [17] | NZN111 | Deletion of PFL and LDH. Overexpressed MAE | Anaerobic, batch | 12.8 | 0.29 | 0.64 | — | Acetate | [18] | AFP111 | Glucose transport by ATP-dependent phosphorylation. Deletion of PFL and LDH | Anaerobic, batch | 12.8 | — | 0.7 | 0.16 g L−1 | Acetate, ethanol | [19] | | | Dual phase aeration, fed-batch | — | 1.21 | 0.96 | 10 (OD600) | Acetate | [20] | AFP111-pyc | AFP111 with overexpressed PYC | Dual-phase aeration, fed-batch | 99.2 | 1.31 | 1.1 | — | Pyruvate, formate | [21] | SBS110MG | Deletion of LDH, PFL Overexpressed PYC | Anaerobic, batch | 15.6 | 0.33 | 0.85 | — | Acetate, formate | [22] | SBS550MG (pHL314) | Deletion of ADH, LDH, ICLR, and ACK-PTA Overexpressed PYC | Anaerobic, fed-batch | 40 | 0.42 | 1.06 | 17 (OD600) | Formate, acetate | [23] | SBS990MG (pHL314) | Deletion of ADHE, LDHA, ACK-PTA Overexpressed PYC | Anaerobic, batch | 15.9 | 0.64 | 1.07 | 9 (OD600) | Formate | [24] | GJT (pHL333, pHL413) | Coexpression of PEPC and pantothenate kinase (PANK) | Anaerobic, batch | 2.05 | 0.085 | 0.1 | — | Lactate | [25] | YBS132 (pHL333, pHL413) | Coexpression of PEPC and PYC Deletion of LDH and ACK-PKA | Anaerobic, batch | 3.4 | 0.14 | 0.2 | — | Acetate, ethanol | [26] | HL51276k | Mutation in the tricarboxylic acid cycle (SDHAB, ICD, ICLR) and acetate pathways (POXB, ACKA-PTA) | Aerobic, batch | 4.61 | 0.06 | 0.43 | — | Pyruvate, acetate | [27] | HL51276k- pepc | HL51276k with overexpressed PEPC | Aerobic, batch | 8 | 0.14 | 0.72 | — | Pyruvate, acetate | [27] | KJ060 | Deletion of LDH, ADHE, ACKA, FOCA, PFLB. Glucose transport by ATP-dependent phosphorylation. PEPC was replaced by the gluconeogenic PEPC | Anaerobic, fed-batch | 86.6 | 0.9 | 0.92 | 2.2 g L−1 | Malate, acetate | [8] | KJ134 | Deletion of LDH, ADHE, FOCA-PFLB, MGSA, POXB, TDCDE, CITF, ASPC, SFCA, PTA-ACKA. Glucose transport by ATP-dependent phosphorylation. PEPC was replaced by the gluconeogenic PEPC | Anaerobic, fed-batch | 71.6 | 0.75 | 1 | 2.3 g L−1 | Acetate, pyruvate, malate | [28] |
| Corynebacterium glutamicum |
| ΔldhA-pCRA717 | Deletion of LDHa. Overexpressed PYC | Micro-aerobic, fed-batch with membrane for cell recycling | 146 | 3.17 | 0.92 | 60 g L−1 | Acetate | [29] |
| Saccharomyces cerevisiae |
| Suc-200 | Deletion of ADH1, ADH2 and GPD1. Overexpressed PCKa, FUMR, FUM, MDH and MAE1 | Aerobic, fed-batch | 34.5 | 0.24 | — | — | — | [30] | Suc-297 | Deletion of ADH1, ADH2 and GPD1 Overexpressed PEPC, NADH-dependent FUMR, FUM, MDH and malic acid transporter protein, PYC | Aerobic, fed-batch | 43 | 0.45 | — | — | — | [30] | Kura | Deletion of SDH1 and FUM1 | Aerobic, batch | 2.32 | 0.005 | 0.015 | — | Ethanol, malate, lactate | [31] | 8D (pRS426T-ICL1-C)
| Deletion of SDH and SER3/SER33 Overexpression of ICL1 | Aerobic, batch | 0.9 | 0.05 g/g | — | — | — | [32] | AH22ura3 Δsdh2Δsdh1Δidh1Δidp1 | Deletion of SDH and IDH | Aerobic, batch | 3.62 | 0.022 | 0.072 | 7.0 g L−1 | Ethanol, glycerol, acetate | [33] |
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