Table 3: Method-validation data for the quantitative determination of acetaminophen by NP-TLC with densitometrya.

Method characteristicResults

Specificity Specific
Range [μg · spot−1]
Linearity [μg · spot−1]A = 2444.4(±68.3) + 7249.8(±63.7) · x
; ; ; ;
Detection limit (DL) [μg · spot−1]0.09
Quantitation limit (QL) [μg · spot−1]0.27

For tablets
Product 1Product 2Product 3

Accuracy
 For 50% acetaminophen added ( ) ; CV = 1.13% ; CV = 0.91% ; CV = 1.29%
 For 100% acetaminophen added ( ) ; CV = 1.21% ; CV = 0.92% ; CV = 1.33%
 For 150% acetaminophen added ( ) ; CV = 0.86% ; CV = 1.12% ; CV = 1.08%

Precision (CV, [%])
Repeatability
 For 0.50  g · spot−1 ( )0.570.850.99
 For 1.00  g · spot−1 ( )0.660.760.78
 For 1.50  g · spot−1 ( )1.000.731.11
Intermediate
 For 0.50  g · spot−1 ( )0.851.411.11
 For 1.00  g · spot−1 ( )1.201.570.99
 For 1.50  g · spot−1 ( )1.131.361.56

Robustness (CV, [%])RobustRobustRobust

: peak area [AU], x: amount [ g · spot−1] of drug analyzed, r: correlation coefficient, R: recovery [%], CV: coefficient of variation [%].