Figure 3: Leptin effect on Tpoh, Aanat, and Hiomt mRNA expression in cultured rat pineal gland. The glands were challenged with leptin (Lep 1 nM) associated with norepinephrine (NE 1 μM). (a) Tryptophan hydroxylase (Tpoh) mRNA expression. (b) Arylalkylamine-N-acetyl transferase (Aanat) mRNA expression. (c) Hydroxyindole-O-methyltransferase (Hiomt) mRNA expression. One-way ANOVA, followed by Bonferroni’s post hoc test. versus control. Data are reported as the number of transcripts per number of ribosomal protein L37a (Rpl37a) molecules. glands/group and each experiment was repeated 3 times.