Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens
Table 1
Comparison of DNA yield and purity extracted using three different methods from various numbers of SNU 790 cell line.
No. of cells
Extraction method
DNA concentration
(ng/μL)
Total DNA yield (ng)
Purity A260/A280
P value*
50 (n = 3)
A
0.124 ± 0.005
6.180 ± 0.38
1.7 ± 0.1
P = 0.001
B
0.067 ± 0.004
3.367 ± 0.39
1.2 ± 0.5
C
0.066 ± 0.006
3.320 ± 0.39
1.3 ± 0.1
100 (n = 3)
A
0.183 ± 0.007
9.153 ± 0.39
1.5 ± 0.1
P = 0.001
B
0.088 ± 0.023
4.380 ± 0.36
1.6 ± 0.1
C
0.085 ± 0.006
4.240 ± 0.29
1.6 ± 0.1
200 (n = 3)
A
0.260 ± 0.008
12.980 ± 0.41
1.6 ± 0.0
P = 0.003
B
0.157 ± 0.011
7.873 ± 0.55
1.8 ± 0.1
C
0.163 ± 0.031
8.165 ± 0.81
1.4 ± 0.0
500 (n = 3)
A
0.598 ± 0.067
29.900 ± 0.74
1.8 ± 0.1
P = 0.000
B
0.246 ± 0.006
12.293 ± 0.87
1.6 ± 0.0
C
0.296 ± 0.020
14.782 ± 0.73
1.7 ± 0.1
1000 (n = 3)
A
1.125 ± 0.089
57.653 ± 5.90
1.7 ± 0.2
P = 0.010
B
0.451 ± 0.031
22.527 ± 2.59
1.8 ± 0.0
C
0.761 ± 0.026
38.033 ± 2.62
1.6 ± 0.2
A: ammonium sulfate DNA extraction method; B: Pinpoint Slide DNA Isolation System; C: TaKaRa DEXPAT. Analysis of variance. Mean value ± standard deviation.
