Figure 2: Assessment of irreversible binding of morpholino-[131I]IPQA to wild-type and mutant EGFR kinase in four types of human nonsmall cell lung carcinoma cell lines. (a) The autoradiography demonstrates the irreversible and covalent bindings of morpholino-[131I]IPQA to the EGFR kinase domain in different cell lines. (b) The same membrane was stained with anti-EGFR kinase antibody. A single band of radiolabeled protein corresponds to the predominate band of ~170 kDa.