Aminopeptidase N (CD13) Is Involved in Phagocytic Processes in Human Dendritic Cells and Macrophages
Figure 1
Phenotypic differences in human monocyte-derived macrophages (MD-Ms) and monocyte-derived DCs (MD-DCs). Freshly isolated human monocytes were incubated in RPMI complete medium containing 1000 U/mL IL-4 and 800 U/mL GM-CSF (for MD-DCs) or complete RPMI medium without cytokines (for MD-Ms) for 7 days. The dot plots (a) show the populations of MD-Ms and MD-DCs obtained after 7 days in culture. Expression of CD14 (b) and CD1a (c) was evaluated using flow cytometry. Histograms correspond to MD-Ms (gray line), MD-DCs (black line), and isotype control (dashed line). MD-Ms were 95% CD14+ and 6% CD1a+. MD-DCs were 7% CD14+ and 84% CD1a+.
