Figure 3: Effect of chemotherapeutic agents on MC540 fluorescence intensity. Cells were incubated with control vehicle or each agent and then harvested at the time when the alive and susceptible population reached a maximum. MC540 was added and assayed as described in Section 2. Data were analyzed by one-way analysis of variance followed by a Dunnett’s post test comparing methotrexate ( ), actinomycin D ( ), paclitaxel ( ), and daunorubicin ( ) to control ( ). The overall effect of treatment was significant ( ) with all treatments differing individually from the control ( ).