ISG15 Inhibits IFN-α-Resistant Liver Cancer Cell Growth
Figure 2
ISG15 overexpression induced ISGylation and ubiquitination and increased p53 levels (a) ISGylation and ubiquitination were detected by western blotting. HepG2 cells treated with IFN-α or transiently transfected with different plasmid were cultured for 48 h, and protein expression was assessed by western blotting. (b) p53 expression was examined by western blotting using anti-p53 antibody in cells treated with different factors for 48 h. (c) Densitometry of Western blotting bands was quantified by using ImageJ software (gray-scale band analysis). Data were analyzed by one-way ANOVA and post hoc LSD test. Results are shown as mean ± SD (). * compared with control HepG2 cells.
