Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis

Table 2

The nucleotide-homology of the transcript-derived fragments (TDFs) with known gene sequences in the database using the BLASTN and BLASTX algorithms along with their expression patterns.

TDF no.	TDF size (bp)	GenBank/gene	Corresponding or related protein	Score (bits)	Identities	𝐸 value
Carbohydrate metabolism
N21-1	130	ABF96013.1	NAD-dependent epimerase/dehydratase family protein	73.2 (178)	32/33 (97%)	2𝑒−14 *
H50-1	195	PsaB	PSI P700 apoprotein A2	200 (108)	108/108 (100%)	3𝑒−48
H51-4	198	YP_654233	PS II protein H	57.8 (138)	26/27 (96%)	2𝑒−09 *
H51-8	138	ABI34757.1	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (Pentameris aspera)	80.5 (197)	34/35 (97%)	3𝑒−17 *
H53-1	495	NP_039432.1	NADH dehydrogenase subunit 2	205 (522)	139/171 (81%)	1𝑒−60 *
H53-2	389	Os10g0527100	Chloroplast ATP synthase a chain precursor	217 (117)	119/120 (99%)	6𝑒−53
H53-3	299	Os08g0472600	Alpha-1, 3-fucosyltransferase	152 (82)	87/89 (98%)	1𝑒−33
H53-6	261	Os10g0527100	Chloroplast ATP synthase a chain precursor	433 (234)	236/237 (99%)	4𝑒−118
H53-7	220	Os07g0662900	4-Alpha-glucanotransferase	76.8 (41)	46/48 (96%)	6𝑒−11
H56-3	229	AAA84588.1	atpB gene product	145 (366)	71/76 (93%)	1𝑒−39 *
H57-3	294	Os10g0527100	Chloroplast ATP synthase a chain precursor	224 (121)	123/124 (99%)	3𝑒−55
H57-4	290	Os10g0527100	Chloroplast ATP synthase a chain precursor	224 (121)	123/124 (99%)	3𝑒−55
H57-6	195	Os01g0881600	Photosystem II reaction center J protein	110 (59)	61/62 (98%)	5𝑒−21
N58-3	270	Os08g0484500	Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplast precursor	207 (112)	116/118 (98%)	3𝑒−50
Protein metabolism
H1-5	227	Os03g0131300	Ubiquitin domain containing protein	283 (153)	159/162 (98%)	3𝑒−73
H51-5	185	AF093630	60S ribosomal protein L21 (RPL21)	298 (161)	168/171 (98%)	1𝑒−77
N53-6	294	Os07g0555200	Eukaryotic translation initiation factor 4G	147 (79)	82/83 (99%)	6𝑒−32
Polyamine metabolism
H57-5	260	Os02g0611200	S-adenosylmethionine decarboxylase proenzyme (AdoMetDC) (SamDC)	169 (91)	96/98 (98%)	1𝑒−38
Amino acid metabolism
H51-10	118	GAD	Glutamate decarboxylase	172 (93)	103/107 (96%)	3𝑒−40
Ribonucleotide metabolism
H3-2-8	102	Os12g0556600	CTP synthase family protein	143 (77)	79/80 (99%)	2𝑒−31
Cellulose synthesis
H46-3	193	Os04g0429600	Cellulose synthase-like protein H1	124 (67)	100/115 (87%)	2𝑒−25
Material transport
N51-17	181	Os12g0166000	Peptidase S59, nucleoporin family protein	189 (102)	111/115 (97%)	6𝑒−45
H51-3	238	Os01g0239200	Phophate translocator	399 (216)	218/219 (99%)	3𝑒−108
N53-1	372	Os08g0517200	Ca^2+-ATPase isoform 9	580 (314)	316/317 (99%)	2𝑒−162
H57-2	304	Os02g0176700	Potential calcium-transporting; ATPase 9, plasma membrane type	505 (273)	282/286 (99%)	9𝑒−140
H59-4	276	Os02g0788800	Amino acid/polyamine transporter II family protein	466 (252),	252/252 (100%)	4𝑒−128
Stress response
H53-8	191	Os07g0186000	Thioredoxin h isoform 1	189 (102)	104/105 (99%)	6𝑒−45
H53-9	186	Os07g0186000	Thioredoxin h isoform 1	187 (101)	104/105 (99%)	2𝑒−44
N60-4	307	Os07g0620200	Heat shock protein DnaJ, N-terminal domain containing protein	403 (218)	218/218 (100%)	3𝑒−109
Signal transduction
H51-6	175	SRP14	Signal recognition particle Subunit 14	279 (151)	156/158 (99%)	3𝑒−72
H51-7	144	SRP14	Signal recognition particle Subunit 14	209 (113)	122/126 (97%)	3𝑒−51
Cell cycle and fate
H4-1	357	RSPSP94	S-phase-specific ribosomal protein	604 (327)	329/330 (99%)	1𝑒−169
N6-1	296	AY436773.1	Putative senescence- associated protein (Pyrus communis)	503 (272)	277/279 (99%)	3𝑒−139
Unclear functional proteins
H3-2-1	200	Os05g0156800	Hypothetical protein	263 (142)	144/145 (99%)	4𝑒−67
N19-2	369	BAH80065.1	Putative retrotransposon protein	173 (438)	97/100 (97%)	4𝑒−48 *
N51-2	436	Os12g0597400	Hypothetical protein	758 (410)	414/416 (99%)	0.0
N51-3	411	Os12g0444500	Hypothetical protein	479 (259)	270/275 (98%)	8𝑒−132
N51-4	399	BAD68598.1	Hypothetical protein	46.6 (109)	29/59 (49%)	5𝑒−04 *
H51-2	250	Os06g0124900	Hypothetical protein	265 (143)	179/194 (92%)	1𝑒−67
H51-9	128	Os01g0750800	Hypothetical protein	174 (94)	102/106 (96%)	1𝑒−40
N53-5	298	AAQ56570.1	Polyprotein	145 (367)	79/125 (63%)	1𝑒−41 *
H53-11	143	Os04g0599650	Tetratricopeptide-like helical domain containing protein	230 (124)	139/145 (96%)	3𝑒−57
H53-12	140	Os04g0599650	Tetratricopeptide-like helical domain containing protein	228 (123)	133/137 (97%)	9𝑒−57
H55-2	169	Os01g0795000	Hypothetical protein	274 (148)	148/148 (100%)	1𝑒−70
H56-4	212	Os03g0685500	Hypothetical protein	172 (93)	93/93 (100%)	7𝑒−40
H56-5	151	Os12g0590400	Hypothetical protein	147 (79)	91/96 (95%)	3𝑒−32
H56-8	173	Os03g0395600	Hypothetical protein	204 (110)	110/110 (100%)	2𝑒−49
N60-5	303	EEC84589.1	Hypothetical protein OsI_31400	90.9 (224)	45/48 (94%)	5𝑒−19 *
N62-8	305	AAT44171.1	Hypothetical protein	73.2 (178)	35/36 (97%)	1𝑒−15 *

The “H” before the numbers of the TDFs represents upregulated expression in high-temperature treated plants, while “N” represents upregulated expression in normal temperature treated plants (downregulated gene under high temperature). All are BLASTN scores except for those marked with “*”, which are BLASTX scores.