TNFα Mediated IL-6 Secretion Is Regulated by JAK/STAT Pathway but Not by MEK Phosphorylation and AKT Phosphorylation in U266 Multiple Myeloma Cells
Figure 5
Effect of NF-κB inhibitors on TNFα induced IL-6 levels and signaling induction of its upstream pathways. U266 cells were serum starved for 8 hr and pretreated with NF-κB inhibitors (10 M Bay 11-7082, 10 M PDTC, 20 M TPCK) for 1 hr. These cells were then stimulated with 1 ng/mL TNFα for 10 min to stimulate IL-6 secretion. Cell supernatants were subjected to determine IL-6 level using ELISA (a), and proteins were prepared for detection of signaling activation using indicated antibodies (b). Data shown are the means ± SEM of three independent experiments.
