BioMed Research International

Methodology Report

Novel One-Step Multiplex PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of -Thalassemia

Table 1

Primers sequences for the -thalassemia and HLA markers included in our method.


Marker	Primer	Dye	Sequence	Size of fragment (bp)

HLA typing
D6S1571	D6S1571F	PET	GGACCTACGCATCTGGTG	160–180
D6S1571	D6S1571R		TGGCTCTAATGGTTACTTTTTACA	160–180
D6S1260	D6S1260F	NED	ACTGCTCCTGGGCATGGTTG	130–160
D6S1260	D6S1260R		GTACATGCCTTTGTTAACATC	130–160
D6S1624	D6S1624F	6FAM	GGAAGTCTTCAGTGGAGAGAGT	200–220
D6S1624	D6S1624R		ACTCCAGGTGTTTGTGGTTT	200–220
MOG-CA	MGCAF	VIC	TCACCTCGAGTGAGTCTCTTT	205–235
MOG-CA	MOGCAR		ACCATGGGTAACTGAAGCAT	205–235
MOG-TAAA	MOG3F	6FAM	GAAATGTGAGAATAAAGGAGA	125–150
MOG-TAAA	MOG3R		GATAAAGGGGAACTACTACA	125–150
D6S265	D6S265F	6FAM	ACGTTCGTACCCATTAACCT	110–125
D6S265	D6S265R		CGAGGTAAACAGCAGAAAGA	110–125
HLAC-CA	HLACCAF	VIC	TCCCTAGTAGCTGGGATTACA	155–175
HLAC-CA	HLACCAR		CGGCAAGAGACTCTGATGA	155–175
MIB	MIBF	6FAM	CCACAGTCTCTATCAGTCCAGA	155–185
MIB	MIBR		TCAGCCTGCTAGCTTATCCT	155–185
D6S273	D6S273F	6FAM	GGCCAAAGTTAAAACCAAAC	135–145
D6S273	D6S273R		GCAACTTTTCTGTCAATCCA	135–145
DRA-CA	DRACAF	VIC	GATACTTTCCTAATTCTCCTCCTTC	120–140
DRA-CA	DRACAR		ATGGAATCTCATCAAGGTCAG	120–140
D6S2443	D6S2443F	6FAM	CCATACCAAAGTAAAACCCAG	150–200
D6S2443	D6S2443R		GAGGATGAAGGGAAATTAGAG	150–200
DQCAR	DQCARF	PET	CTTGGCCAATCAGAATCTTT	150–175
DQCAR	DQCARR		CTGCATTTCTCTTCCTTATCAC	150–175
TAP1-CA	TAP1CAF	PET	GGACAATATTTTGCTCCTGA	195–215
TAP1-CA	TAP1CAR		TCATACATCTGCTTTGATCTCC	195–215
RING3-CA	RING3CAF	6FAM	GGGCCGCAGTTTAAGTAAC	125–135
RING3-CA	RING3CAR		TGTTAGGTCAGAACCACAGAA	125–135
D6S1560	D6S1560F	6FAM	CTCCAGTCCCCACTGC	225–250
D6S1560	D6S1560R		CCCAAGGCCACATAGC	225–250
D6S1583	D6S1583F	VIC	GCCCCTAACCTGCTTCTACTGA	150–200
D6S1583	D6S1583R		GCAGATGGCCCCACTGAC	150–200
D6S1618	D6S1618F	NED	GGCCTGAGCAGTGCAT	130–170
D6S1618	D6S1618R		TGATTCCTAATCTGCGGG	130–170
D6S1611	D6S1611F	PET	GAGCAAGACTCCATCTCAAA	225–250
D6S1611	D6S1611R		ACCTAAGTTCTCTGAAGGGC	225–250
D6S1610	D6S1610F	PET	CCTGGTGAGATAGATGCTTG	100–140
D6S1610	D6S1610R		ATTTCCAGCAGAGCCTTG	100–140
D6S1552	D6S1552F	VIC	AGCCTGAACGACAGAACAAG	160–200
D6S1552	D6S1552R		CTGCTTAACTTAGATCTTTGGTAT	160–200
-thalassemia locus
D11S4181	D11S4181F	6FAM	AAGCTTCCTTCACATTCTTACAG	200–225
D11S4181	D11S4181R		GAACTGAGACCAAGAACATTATTCC	200–225
D11S2351	D11S2351F	6FAM	GGGCACCTGTAATCCCA	150–180
D11S2351	D11S2351R		AGGAGTCACTGGATCTACTC	150–180
D11S1871	D11S1871F	PET	AAGAAGTTGCCCTGATGTCT	160–200
D11S1871	D11S1871R		TAAAAGGAGCTGAATGCACA	160–200
D11S4891	D11S4891F	6FAM	GGAAATGGACCTCTGTCTC	75–100
D11S4891	D11S4891R		CTTTTATTCCAGCCCCAC	75–100
D11S1760	D11S1760F	PET	GATCTCAAGTGTTTCCCCAC	75–100
D11S1760	D11S1760R		AAACGATGTCTGTCCACTCA	75–100
D11S1338	D11S1338F	6FAM	GACGGTTTAACTGTATATCTAAGAC	250–280
D11S1338	D11S1338R		TAATGCTACTTATTTGGAGTGTG	250–280