Novel One-Step Multiplex PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of 𝜷-Thalassemia

Table 2

Results of the clinical PGD-HLA cycles.

PGD	Cycle 1	Cycle 2
Number of oocytes retrieved	20	25
MII oocytes	17	22
2-pronuclei zygotes	14	18
Embryos biopsied	11	17
Affected	2	8
Carriers	7	3
Noncarriers	2	6
HLA nonidentical	9	14
HLA-identical	2	3
HLA nonidentical and affected	1	7
HLA nonidentical and carriers	6	1
HLA nonidentical and noncarriers	2	6
HLA-identical and affected	1	1
HLA-identical and carriers	1	2
HLA-identical and noncarriers	0	0
Embryos transferred	1	1
Ongoing pregnancy	No	Yes