Methodology Report

Novel One-Step Multiplex PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of -Thalassemia

Table 3

Combinations of markers successfully amplified with our one-step multiplex PCR protocol on a single cell, after the update of the method.

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5′Region3′RegionUpstream HLAAHLAA-HLABHLAB-HLADRAHLADRA-HLADQB1Downstream DQB1

1D11S1338 D11S2351 D6S1624 HLAC-CA MIB DQCAR RING3-CA
2D11S1338 D11S2351 MOG-CA D6S265 D6S273 DRA-CA TAP1A-CA
3D11S4891 D11S4181 D6S1624 HLAC-CA MIB DRA-CA TAP1A-CA
4D11S1760 D11S4181 MOG-CA D6S265 D6S273 DQCAR RING3-CA
5D11S4891 D11S1871 D6S1571 D6S265 MIB D6S2443 D6S1583
6D11S1760 D11S1871 MOG-TAAA HLAC-CA D6S273 D6S2443 D6S1610