Different Mechanisms of Inflammation Induced in Virus and Autoimmune-Mediated Models of Multiple Sclerosis in C57BL6 Mice

Figure 3

Brain inflammation in EAE and RSA59-infected mice. EAE ((a)–(d)): mid-sagittal sections of brains (5 μm thick) at early and late phases after immunization were stained immunohistochemically for CD45 (LCA). ((a), (b)) day 9 after immunization. ((c), (d)): day 30 post immunization. There is mostly leptomeningeal (surface) and deep cerebellar white matter LCA immunoreactivity at early phases that decrease dramatically but remains in the same locations at late time points. ((a), (c)) Scanned images. ((b), (d)) Higher magnification of corresponding deep cerebellar white matter (100x). RSA59-infection ((e)–(h)) mid-sagittal sections of brains (5 μm thick) at early and late phases after inoculation were stained immunohistochemically with CD45 (LCA). ((e), (f)) Day 7. ((g), (h)) Day 30. At early phases post-inoculation, there are both white and gray matters’ infiltration by LCA immunoreactive cells that diminishes in intensity over time and is mainly observed in deep cerebellar white matter at day 30. ((e), (g)) Scanned images. ((f), (h)) Higher magnification of corresponding deep cerebellar white matter (100x). Black lines in (e) and (g) show areas magnified in (f) and (h).