Assessing Competence of Broccoli Consumption on Inflammatory and Antioxidant Pathways in Restraint-Induced Models: Estimation in Rat Hippocampus and Prefrontal Cortex

Figure 4

Western blot analysis to measure the expression of inflammatory proteins, nuclear NF-κB p65, and TNF-α in the hippocampus and prefrontal cortex tissues derived after 6 hours of restraint-induced stress in experimental groups. (a) Immunoblot bands of nuclear NF-κB p65 and TNF-α, as well as their relevant lamin B2 and β-actin bands. ((b) and (c)) The densities of corresponding bands were measured and the ratios to lamin B2 and β-actin were calculated and represented as arbitrary units on the graphs for each experimental group (𝑛=6). Bars indicate the mean ± SEM; one-way ANOVA; *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001.

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