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BioMed Research International
Volume 2013, Article ID 597863, 11 pages
Research Article

Application of a Low-Level Laser Therapy and the Purified Protein from Natural Latex (Hevea brasiliensis) in the Controlled Crush Injury of the Sciatic Nerve of Rats: A Morphological, Quantitative, and Ultrastructural Study

1Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, 2415 Avenida Professor Lineu Prestes, 05508-900 Sao Paulo, SP, Brazil
2Department of Morphology, Physiology and Basic Pathology, Faculty of Dentistry of Ribeirao Preto, University of Sao Paulo, S/N Avenida do Café, 14040-904 Ribeirao Preto, SP, Brazil
3Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, 3900 Avenida Bandeirantes, 14049-900 Ribeirao Preto, SP, Brazil
4Dental School, University of La Frontera, 1145 Avenida Francisco Salazar, 01145 Temuco, Chile

Received 10 April 2013; Revised 5 June 2013; Accepted 5 June 2013

Academic Editor: Richard Tucker

Copyright © 2013 Fernando José Dias et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study analyzed the effects of a low-level laser therapy (LLLT, 15 J/cm2, 780 nm wavelength) and the natural latex protein (P1, 0.1%) in sciatic nerve after crush injury (15 Kgf, axonotmesis) in rats. Sixty rats (male, 250 g) were allocated into the 6 groups ( ): CG—control group; EG—nerve exposed; IG—injured nerve without treatment; LG—crushed nerve treated with LLLT; PG—injured nerve treated with P1; and LPG—injured nerve treated with LLLT and P1. After 4 or 8 weeks, the nerve samples were processed for morphological, histological quantification and ultrastructural analysis. After 4 weeks, the myelin density and morphological characteristics improved in groups LG, PG, and LPG compared to IG. After 8 weeks, PG, and LPG were similar to CG and the capillary density was higher in the LG, PG, and LPG. In the ultrastructural analysis the PG and LPG had characteristics that were similar to the CG. The application of LLLT and/or P1 improved the recovery from the nerve crush injury, and in the long term, the P1 protein was the better treatment used, since only the application of LLLT has not reached the same results, and these treatments applied together did not potentiate the recovery.