Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction

Figure 6

Flow cytometry showed DNA content in EBs derived from iPS cells with RA or testosterone in vitro. EBs were dispersed as single cells and stained with the DNA-binding dye Hoechst 33342 to reveal DNA content, and the cell debris was removed by setting the gates. M1, haploid cells; M2, diploid cells; M3, tetraploid cells. C: control group, M1 (1.15%), M2 (77.72%), and M3 (9.31%); RA: RA induction group, M1 (8.31%), M2 (61.69%), and M3 (12.98%); T: testosterone induction group, M1 (0.24%), M2 (81.27%), and M3 (10.17%); RA/T: RA and testosterone induction group, M1 (2.56%), M2 (74.49%), and M3 (8.86%); testis: testicular cells, M1 (59.67%), M2 (15.07%), and M3 (25.68%).