Research Article
Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids
Table 2
Results of the qualitative and quantitative analyses of 2DE performed in keloid fibroblasts and adjacent normal fibroblasts.
| Spot no. | Identified protein | Accession number | Sequence coverage (%) | Theoretical Mr (KDa)/pI | Measured Mr (KDa)/pI | Est’d Z | Characteristics |
| B8 | Heat shock 70 kDa protein 9B | NP-004125 | 23 | 5.7/70 | 5.9/74.02 | 1.68 | Keloid-specific | B12 | Vimentin | A25074 | 20 | 4.9/44 | 5.1/53.7 | 1.79 | Keloid-specific | B16 | DnaK-type molecular chaperone HSPA1L | A29160 | 24 | 5.7/70 | 5.4/70.21 | 2.16 | Keloid-specific | B19 | Dihydropyrimidinase-like 2 | NP-001377 | 20 | 6.5/60 | 6/62.84 | 1.67 | Keloid-specific | B-IN1 | Crocalbin-like protein | AAF76141 | 38 | 4.3/40 | 4.4/34.97 | 1.71 | Upregulated in keloid | | Calumenin | AAK72908 | 36 | | 4.4/37.26 | | Upregulated in keloid | B-IN2 | Vimentin | NP-003371 | 29 | 4.9/45 | 5.1/53.74 | 2.3 | Upregulated in keloid | B-IN3 | BiP protein | AAF13605 | 23 | 5/75 | 5.2/71.06 | 2.15 | Upregulated in keloid | A11 | Proteasome alpha 6 subunit | NP-002782 | 27 | 6.6/28 | 6.3/27.95 | 2.37 | Does not exist in keloid | A-DE1 | Tropomyosin 1 | A23562 | 28 | 5/40 | 4.6/33.04 | 2.34 | Upregulated in keloid | A-DE3 | Heat shock 70 kDa protein 1A | NP-005336 | 27 | 5.7/70 | 5.5/70.38 | 2.32 | Upregulated in keloid | A-IN1 | Vimentin | AAA61279 | 39 | 5/55 | 5/53.77 | 2.35 | Downregulated in keloid | A-IN2 | Vimentin | NP-003371 | 37 | 5/55 | 5.1/53.74 | 2.39 | Downregulated in keloid | A-IN4 | Vimentin | A25074 | 30 | 5.1/55 | 5.1/53.7 | 2.35 | Downregulated in keloid | A-IN6 | Tropomyosin 4-anaplastic lymphoma kinase fusion protein | AAK17926 | 12 | 5/40 | 4.9/36.78 | 2.06 | Downregulated in keloid | A-IN11 | Endoplasmic reticulum protein 29 precursor | NP-006808 | 28 | 6/29 | 6.8/29.05 | 2.09 | Downregulated in keloid | A-IN12 | Heat shock 27 kDa protein 1 | NP-001531 | 33 | 6.1/26 | 6/22.84 | 2.35 | Downregulated in keloid |
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