Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids

Table 2

Results of the qualitative and quantitative analyses of 2DE performed in keloid fibroblasts and adjacent normal fibroblasts.

Spot no.	Identified protein	Accession number	Sequence coverage (%)	Theoretical Mr (KDa)/pI	Measured Mr (KDa)/pI	Est’d Z	Characteristics
B8	Heat shock 70 kDa protein 9B	NP-004125	23	5.7/70	5.9/74.02	1.68	Keloid-specific
B12	Vimentin	A25074	20	4.9/44	5.1/53.7	1.79	Keloid-specific
B16	DnaK-type molecular chaperone HSPA1L	A29160	24	5.7/70	5.4/70.21	2.16	Keloid-specific
B19	Dihydropyrimidinase-like 2	NP-001377	20	6.5/60	6/62.84	1.67	Keloid-specific
B-IN1	Crocalbin-like protein	AAF76141	38	4.3/40	4.4/34.97	1.71	Upregulated in keloid
	Calumenin	AAK72908	36		4.4/37.26		Upregulated in keloid
B-IN2	Vimentin	NP-003371	29	4.9/45	5.1/53.74	2.3	Upregulated in keloid
B-IN3	BiP protein	AAF13605	23	5/75	5.2/71.06	2.15	Upregulated in keloid
A11	Proteasome alpha 6 subunit	NP-002782	27	6.6/28	6.3/27.95	2.37	Does not exist in keloid
A-DE1	Tropomyosin 1	A23562	28	5/40	4.6/33.04	2.34	Upregulated in keloid
A-DE3	Heat shock 70 kDa protein 1A	NP-005336	27	5.7/70	5.5/70.38	2.32	Upregulated in keloid
A-IN1	Vimentin	AAA61279	39	5/55	5/53.77	2.35	Downregulated in keloid
A-IN2	Vimentin	NP-003371	37	5/55	5.1/53.74	2.39	Downregulated in keloid
A-IN4	Vimentin	A25074	30	5.1/55	5.1/53.7	2.35	Downregulated in keloid
A-IN6	Tropomyosin 4-anaplastic lymphoma kinase fusion protein	AAK17926	12	5/40	4.9/36.78	2.06	Downregulated in keloid
A-IN11	Endoplasmic reticulum protein 29 precursor	NP-006808	28	6/29	6.8/29.05	2.09	Downregulated in keloid
A-IN12	Heat shock 27 kDa protein 1	NP-001531	33	6.1/26	6/22.84	2.35	Downregulated in keloid