Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
Figure 6
Neural differentiation of DMS/PCs. (a) Control cells. (b) After 1 week of induction, neural-like cells with a multipolar spindle-like shape were observed. (c) After 2 weeks of induction, neural-like cells were observed as indicated by the arrow. (d–i) Double immunofluorescence staining showed that (d) nestin (red) and (e) β-III tubulin (green) were positive in the induced cells. (f) Merged image of d and e. (g) NF (red) and SYP (h) were positive in the inducted group. (i) Merged image of g and h. Cells were counterstained with DAPI (blue) (scale bar: 100 μm). (j) After induction for 14 days, expression of neural-specific genes nestin and NF was detected by RT-PCR in the induced group, whereas these genes were not expressed in the control group. Lane 1: nestin was positive in the inducted group; lane 2: nestin was positive in the control group; line 3: NF was positive in the inducted group; lane 4 NF was negative in the control group; lanes 5 and 6: GAPDH served as the internal control.
