Relative mRNA levels for ALP in MC3T3-E1 osteoblast-like cells on HAP, HAP—30% PA, and HAP—30% PA—12 h at 14, 21, and 28 days after obtaining a confluent cell monolayer. At 14 days, there was no mRNA expression on HAP, but the expression on HAP—30% PA—12 h and HAP—30% PA was estimated in sequence. At 21 days, the expression of ALP on HAP was estimated, and the expression on HAP, HAP—30% PA, and HAP—30% PA—12 h was increased at 28 days. There were no statistically significant differences among expressions at any time ().
