Figure 1: Acetaminophen (APAP) induces dose-dependent lethality and LTB4 production in the liver. (a) WT mice were treated with APAP (0.3, 1, 3, and 6 g/kg) or saline per oral, and lethality was assessed. The lethality induced by APAP was monitored at 6 h intervals during 72 h. , representative of three separate experiments. (b) WT mice were treated with APAP (3 g/kg, per oral) or saline, and after 12 h liver samples were collected for the determination of LTB4 levels by ELISA. Values are mean S.E.M., , representative of two separate experiments. * compared to saline group, Student’s t-test.