5-Lipoxygenase Deficiency Reduces Acetaminophen-Induced Hepatotoxicity and Lethality

Figure 1

Acetaminophen (APAP) induces dose-dependent lethality and LTB₄ production in the liver. (a) WT mice were treated with APAP (0.3, 1, 3, and 6 g/kg) or saline per oral, and lethality was assessed. The lethality induced by APAP was monitored at 6 h intervals during 72 h. 𝑛=10, representative of three separate experiments. (b) WT mice were treated with APAP (3 g/kg, per oral) or saline, and after 12 h liver samples were collected for the determination of LTB₄ levels by ELISA. Values are mean ± S.E.M., 𝑛=5, representative of two separate experiments. *𝑃<0.05 compared to saline group, Student’s t-test.

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