5-Lipoxygenase Deficiency Reduces Acetaminophen-Induced Hepatotoxicity and Lethality
Figure 3
Acetaminophen (APAP) induces 5-LO-dependent histopathological changes in the liver. WT and 5-LO−/− mice were treated with APAP (3 g/kg) or saline per oral, and after 12 h liver samples were collected and processed for histopathology analysis. The degree of liver necrosis (a) ( for saline groups, for WT APAP group, and for 5-LO−/− APAP group) and inflammation (b) (-5 for saline groups, for WT APAP group, and for 5-LO−/− APAP group) were assessed. *P < 0.05 compared to saline-treated WT and 5-LO−/− mice and # compared to APAP-treated WT mice. Kruskal-Wallis test was followed by Dunn’s multiple comparison test. (c–f) Representative images of histopathological changes in the liver (H&E 40). (c) WT mice treated with saline, (d) 5-LO−/− mice treated with saline, (e) WT mice treated with APAP, and (f) 5-LO−/− mice treated with APAP.
