5-Lipoxygenase Deficiency Reduces Acetaminophen-Induced Hepatotoxicity and Lethality
Figure 7
Acetaminophen (APAP) induces hepatic oxidative stress in a 5-LO-dependent manner. WT and 5-LO−/− mice were treated with APAP (3 g/kg) or saline per oral, and after 12 h liver samples were collected to determine superoxide anion production (nitroblue tetrazolium (NBT) reduction) (a), lipid peroxidation (thiobarbituric acid reactive substances (TBARS)) levels (b), (c) reduced glutathione (GSH) levels, and (d) antioxidant capacity by 2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonate; ABTS) assay. Values are mean S.E.M., , representative of two separate experiments. * compared to saline-treated WT and 5-LO−/− mice and # compared to APAP-treated WT mice. One-way ANOVA was followed by Bonferroni’s multiple comparison test.
