BioMed Research International

Research Article

Bovine Papillomavirus Clastogenic Effect Analyzed in Comet Assay

Table 1

(a) Results of molecular diagnosis of calves (negative control) and asymptomatic and symptomatic adult cattle, showing the BPV type, the frequency of nucleoids observed per class, and the comet score. (b) Kruskal-Wallis statistic to compare the clastogenicity among calves, asymptomatic and symptomatic cattle, from the comet score. (c) Comparison between comet score observed in monoinfected and coinfected bovines through Mann-Whitney test.

(a)


Controls	Clinical	Virus type	Class of nucleoids			Score
Controls	Clinical	Virus type	0	1	2	Score

1	Asymptomatic	—	93	4	3	10
2	Asymptomatic	—	92	5	3	11
3	Asymptomatic	—	96	1	3	7
4	Asymptomatic	—	90	8	2	12
5	Asymptomatic	—	95	3	2	7

Infected bovines

6	Asymptomatic	BPV-1	15	60	25	110
7	Asymptomatic	BPV-2	57	36	7	50
8	Asymptomatic	BPV-1	75	19	6	31
9	Asymptomatic	BPV-1, -2	43	17	40	97
10	Asymptomatic	BPV-1	73	19	8	35
11	Asymptomatic	BPV-1, -2	47	26	27	80
12	Asymptomatic	BPV-1	38	26	36	98
13	Asymptomatic	BPV-1, -2	62	13	25	63
14	Asymptomatic	BPV-2	72	21	7	35
15	Asymptomatic	BPV-1, -2	63	12	25	62
16	Asymptomatic	BPV-1, -2	58	8	34	76
17	Asymptomatic	BPV-1, -2	40	42	18	78
18	Symptomatic	BPV-1, -2	61	30	9	48
19	Symptomatic	BPV-2	22	13	65	143
20	Symptomatic	BPV-1, -2	65	12	23	58
21	Symptomatic	BPV-1, -2	57	21	22	65
22	Symptomatic	BPV-1, -2, -4	31	31	38	107
23	Symptomatic	BPV-1	89	6	5	16
24	Symptomatic	BPV-1	92	2	6	14
25	Symptomatic	BPV-1	69	25	6	37
26	Symptomatic	BPV-1	41	10	49	108
27	Symptomatic	BPV-1, -2, -4	70	21	9	39
28	Symptomatic	BPV-1, -2, -4	65	26	9	44
29	Symptomatic	BPV-2	87	8	5	18
30	Symptomatic	BPV-1, -2	0	66	34	134
31	Symptomatic	BPV-1, -2	60	31	9	49
32	Symptomatic	BPV-2	52	23	25	73
33	Symptomatic	BPV-1, -2	72	9	19	47
34	Symptomatic	BPV-1, -2	91	7	2	11
35	Symptomatic	BPV-1, -2	74	15	11	37
36	Symptomatic	BPV1, -2, -4	82	13	5	23
37	Symptomatic	BPV-1, -2	36	22	42	106

(b)


Groups	Average post	Z calculated	P value

Calves and asymptomatic	3.300	3.5348	<0.05
Calves and symptomatic	23.666	3.1097	<0.05
Asymptomatic and symptomatic	20.125	0.8961	n.s.

, , of calves = 16.5, of asymptomatic = 284.0 and of symptomatic = 402.5.

(c)


Monoinfected sample	Score	Coinfected sample	Score

6	110	9	97
7	50	11	80
8	31	13	63
10	35	15	62
12	98	16	76
14	35	17	78
19	143	18	48
23	16	20	20
24	14	21	21
25	37	22	22
26	108	27	39
29	18	28	44
32	74	30	134
—	—	31	49
—	—	33	47
—	—	34	11
—	—	35	37
—	—	36	23
—	—	37	106

Mann-Whitney U test results: , , P value = 0.21.