CpG ODN and ISCOMATRIX Adjuvant: A Synergistic Adjuvant Combination Inducing Strong T-Cell IFN- Responses
Figure 5
(a) BALB/c mice (/group) were immunized on days 0 and 28 by IM injection of 1 μg HBsAg either alone (none) or combined with CpG (10 μg), ISCOMATRIX (IMX) (1 μg), or CpG (10 μg) + ISCOMATRIX (1 μg). Spleens were removed 4 wks after second immunization and IFN-γ secretion was measured by ELISA in antigen-restimulated splenocytes culture supernatants. (b) C57Bl/6 mice (n = 10/group) were immunized on days 0, 14, and 21 by IM injection of 10 μg OVA either alone (none) or combined with CpG (10 μg), ISCOMATRIX (1 μg), or CpG (10 μg) + ISCOMATRIX (1 μg). Spleens were removed 1 wk after final immunization and IFN-γ secretion was measured by ELISPOT in antigen-restimulated splenocytes culture supernatants. (c) BALB/c mice (/group) received a single IM immunization of 1 g influenza. A haemagglutinin antigen (HA) either alone (none) or combined with CpG (10 μg), ISCOMATRIX (1 μg) or CpG (10 μg) + ISCOMATRIX (1 μg). Spleens were removed 4 wks after immunization and IFN-γ secretion was measured by ELISA in antigen-restimulated splenocytes culture supernatants. Representative data from one of five independent experiments is shown for HBsAg and OVA and one of two independent experiments for HA in each graph.
