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BioMed Research International
Volume 2013, Article ID 638085, 7 pages
http://dx.doi.org/10.1155/2013/638085
Methodology Report

Noninvasive Measurement of Murine Hepatic Acetyl-CoA 13C-Enrichment Following Overnight Feeding with 13C-Enriched Fructose and Glucose

1Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, Coimbra 3004-517, Portugal
2Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390-8568, USA
3Department of Chemistry, University of Coimbra, Coimbra 3004-535, Portugal
4APDP-Portuguese Diabetes Association, Lisbon 1250-261, Portugal

Received 2 April 2013; Accepted 14 May 2013

Academic Editor: Yutaka Yata

Copyright © 2013 Filipa Carvalho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. L. Tappy and K. Le, “Metabolic effects of fructose and the worldwide increase in obesity,” Physiological Reviews, vol. 90, no. 1, pp. 23–46, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. M. J. Dekker, Q. Su, C. Baker, A. C. Rutledge, and K. Adeli, “Fructose: a highly lipogenic nutrient implicated in insulin resistance, hepatic steatosis, and the metabolic syndrome,” American Journal of Physiology, vol. 299, no. 5, pp. E685–E694, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. V. T. Samuel, “Fructose induced lipogenesis: from sugar to fat to insulin resistance,” Trends in Endocrinology and Metabolism, vol. 22, no. 2, pp. 60–65, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. E. J. Parks, L. E. Skokan, M. T. Timlin, and C. S. Dingfelder, “Dietary sugars stimulate fatty acid synthesis in adults,” The Journal of Nutrition, vol. 138, no. 6, pp. 1039–1046, 2008. View at Google Scholar · View at Scopus
  5. W. C. Schumann, I. Magnusson, V. Chandramouli, K. Kumaran, J. Wahren, and B. R. Landau, “Metabolism of [2-14C]acetate and its use in assessing hepatic krebs cycle activity and gluconeogenesis,” The Journal of Biological Chemistry, vol. 266, no. 11, pp. 6985–6990, 1991. View at Google Scholar · View at Scopus
  6. M. K. Hellerstein, M. Christiansen, S. Kaempfer et al., “Measurement of de novo hepatic lipogenesis in humans using stable isotopes,” The Journal of Clinical Investigation, vol. 87, no. 5, pp. 1841–1852, 1991. View at Google Scholar · View at Scopus
  7. S. Bingham and J. H. Cummings, “The use of 4-aminobenzoic acid as a marker to validate the completeness of 24 h urine collections in man,” Clinical Science, vol. 64, no. 6, pp. 629–635, 1983. View at Google Scholar · View at Scopus
  8. L. Wang, W. Huang, and H. Tai, “Simultaneous determination of p-aminobenzoic acid and its metabolites in the urine of volunteers, treated with p-aminobenzoic acid sunscreen formulation,” Journal of Pharmaceutical and Biomedical Analysis, vol. 43, no. 4, pp. 1430–1436, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. Y. Zhang, K. C. Agarwal, M. Beylot et al., “Nonhomogeneous labeling of liver extra-mitochondrial acetyl-CoA. Implications for the probing of lipogenic acetyl-CoA via drug acetylation and for the production of acetate by the liver,” The Journal of Biological Chemistry, vol. 269, no. 15, pp. 11025–11029, 1994. View at Google Scholar · View at Scopus
  10. L. A. Bertocci, J. G. Jones, C. R. Malloy, R. G. Victor, and G. D. Thomas, “Oxidation of lactate and acetate in rat skeletal muscle: analysis by 13C-nuclear magnetic resonance spectroscopy,” Journal of Applied Physiology, vol. 83, no. 1, pp. 32–39, 1997. View at Google Scholar · View at Scopus
  11. C. R. Malloy, J. R. Thompson, F. M. H. Jeffrey, and A. D. Sherry, “Contribution of exogenous substrates to acetyl coenzyme A: measurement by 13C NMR under non-steady-state conditions,” Biochemistry, vol. 29, no. 29, pp. 6756–6761, 1990. View at Publisher · View at Google Scholar · View at Scopus
  12. F. M. H. Jeffrey, C. J. Storey, A. D. Sherry, and C. R. Malloy, “13C isotopomer model for estimation of anaplerotic substrate oxidation via acetyl-CoA,” American Journal of Physiology, vol. 271, no. 4, pp. E788–E799, 1996. View at Google Scholar · View at Scopus
  13. M. K. Hellerstein, R. A. Neese, P. Linfoot, M. Christiansen, S. Turner, and A. Letscher, “Hepatic gluconeogenic fluxes and glycogen turnover during fasting in humans. A stable isotope study,” The Journal of Clinical Investigation, vol. 100, no. 5, pp. 1305–1319, 1997. View at Google Scholar · View at Scopus
  14. I. Magnusson, W. C. Schumann, G. E. Bartsch et al., “Noninvasive tracing of Krebs cycle metabolism in liver,” The Journal of Biological Chemistry, vol. 266, no. 11, pp. 6975–6984, 1991. View at Google Scholar · View at Scopus
  15. K. Ekberg, V. Chandramouli, K. Kumaran, W. C. Schumann, J. Wahren, and B. R. Landau, “Gluconeogenesis and glucuronidation in liver in vivo and the heterogeneity of hepatocyte function,” The Journal of Biological Chemistry, vol. 270, no. 37, pp. 21715–21717, 1995. View at Publisher · View at Google Scholar · View at Scopus
  16. F. Diraison, V. Large, H. Brunengraber, and M. Beylot, “Non-invasive tracing of liver intermediary metabolism in normal subjects and in moderately hyperglycaemic NIDDM subjects. Evidence against increased gluconeogenesis and hepatic fatty acid oxidation in NIDDM,” Diabetologia, vol. 41, no. 2, pp. 212–220, 1998. View at Publisher · View at Google Scholar · View at Scopus
  17. V. Large, H. Brunengraber, M. Odeon, and M. Beylot, “Use of labeling pattern of liver glutamate to calculate rates of citric acid cycle and gluconeogenesis,” American Journal of Physiology, vol. 272, no. 1, pp. E51–E58, 1997. View at Google Scholar · View at Scopus
  18. C. Barosa, M. Almeida, M. M. Caldeira, F. Gomes, and J. G. Jones, “Contribution of proteolytic and metabolic sources to hepatic glutamine by2H NMR analysis of urinary phenylacetylglutamine2H-enrichment from 2H2O,” Metabolic Engineering, vol. 12, no. 1, pp. 53–61, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. J. G. Jones, P. Garcia, C. Barosa, T. C. Delgado, and L. Diogo, “Hepatic anaplerotic outflow fluxes are redirected from gluconeogenesis to lactate synthesis in patients with Type 1a glycogen storage disease,” Metabolic Engineering, vol. 11, no. 3, pp. 155–162, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. J. G. Jones, A. Fagulha, C. Barosa et al., “Noninvasive analysis of hepatic glycogen kinetics before and after breakfast with deuterated water and acetaminophen,” Diabetes, vol. 55, no. 8, pp. 2294–2300, 2006. View at Publisher · View at Google Scholar · View at Scopus
  21. R. A. Neese, J.-. Schwarz, D. Faix et al., “Gluconeogenesis and intrahepatic triose phosphate flux in response to fasting or substrate loads. Application of the mass isotopomer distribution analysis technique with testing of assumptions and potential problems,” The Journal of Biological Chemistry, vol. 270, no. 24, pp. 14452–14463, 1995. View at Publisher · View at Google Scholar · View at Scopus
  22. T. H. Van Dijk, F. H. Van Der Sluijs, C. H. Wiegman et al., “Acute inhibition of hepatic glucose-6-phosphatase does not affect gluconeogenesis but directs gluconeogenic flux toward glycogen in fasted rats. A pharmacological study with the chlorogenic acid derivative S4048,” The Journal of Biological Chemistry, vol. 276, no. 28, pp. 25727–25735, 2001. View at Publisher · View at Google Scholar · View at Scopus
  23. R. H. J. Bandsma, C. H. Wiegman, A. W. Herling et al., “Acute inhibition of glucose-6-phosphate translocator activity leads to increased de novo lipogenesis and development of hepatic steatosis without affecting VLDL production in rats,” Diabetes, vol. 50, no. 7–12, pp. 2591–2597, 2001. View at Google Scholar · View at Scopus
  24. L. C. Hudgins, M. Hellerstein, C. Seidman, R. Neese, J. Diakun, and J. Hirsch, “Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet,” The Journal of Clinical Investigation, vol. 97, no. 9, pp. 2081–2091, 1996. View at Google Scholar · View at Scopus
  25. F. Diraison, C. Pachiaudi, and M. Beylot, “Measuring lipogenesis and cholesterol synthesis in humans with deuterated water: use of simple gas chromatographic mass spectrometric techniques,” Journal of Mass Spectrometry, vol. 32, pp. 81–86, 1997. View at Google Scholar
  26. M. F. Chong, B. A. Fielding, and K. N. Frayn, “Mechanisms for the acute effect of fructose on postprandial lipemia,” American Journal of Clinical Nutrition, vol. 85, no. 6, pp. 1511–1520, 2007. View at Google Scholar · View at Scopus
  27. F. M. H. Jeffrey, C. J. Storey, A. D. Sherry, and C. R. Malloy, “13C isotopomer model for estimation of anaplerotic substrate oxidation via acetyl-CoA,” American Journal of Physiology, vol. 271, no. 4, pp. E788–E799, 1996. View at Google Scholar · View at Scopus
  28. A. D. Sherry, F. M. H. Jeffrey, and C. R. Malloy, “Analytical solutions for 13C isotopomer analysis of complex metabolic conditions: substrate oxidation, multiple pyruvate cycles, and gluconeogenesis,” Metabolic Engineering, vol. 6, no. 1, pp. 12–24, 2004. View at Publisher · View at Google Scholar · View at Scopus
  29. C. R. Malloy, A. D. Sherry, and F. M. H. Jeffrey, “Evaluation of carbon flux and substrate selection through alternate pathways involving the citric acid cycle of the heart by 13C NMR spectroscopy,” The Journal of Biological Chemistry, vol. 263, no. 15, pp. 6964–6971, 1988. View at Google Scholar · View at Scopus