Noninvasive Measurement of Murine Hepatic Acetyl-CoA ¹³C-Enrichment Following Overnight Feeding with ¹³C-Enriched Fructose and Glucose

Table 1

Hepatic acetyl-CoA ¹³C-isotopomer abundances and fractional contributions of substrates to hepatic acetyl-CoA appearance in naturally feeding mice with their drinking water supplemented by two different concentrations of a 99% [1-¹³C]glucose/50% [U-¹³C]fructose mixture. *The estimated glucose contributions represent upper and lower limits based on the assumption that [1-¹³C]acetyl CoA was either entirely derived from [1-¹³C]glucose or from [U-¹³C]fructose, respectively.

Drinking water sugar levels (% w/v)	Acetyl ^13C-isotopomer excess enrichments			Substrate contributions to acetyl CoA (%)
	[1-^13C]acetyl	[2-^13C]acetyl	[1,2-^13C2]acetyl	Glucose*	Fructose	Endogenous
5% Fructose and 5% glucose (𝑛 = 4)	0.4 ± 0.1	1.1 ± 0.1	2.0 ± 0.2	3.0 ± 0.2	4.0 ± 0.4	93.0 ± 0.4
				1.4 ± 0.1	4.0 ± 0.4	94.6 ± 0.4
17.5% Fructose and 17.5% glucose (𝑛 = 4)	1.1 ± 0.1	2.6 ± 0.1	5.3 ± 0.3	7.4 ± 0.2	10.6 ± 0.6	82.2 ± 0.4
				3.0 ± 0.1	10.6 ± 0.6	86.6 ± 0.4