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BioMed Research International
Volume 2013 (2013), Article ID 642964, 15 pages
http://dx.doi.org/10.1155/2013/642964
Research Article

Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

1Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin 640, Taiwan
2Department of Nephrology, Chi-Mei Medical Center, Tainan 710, Taiwan
3Department of Sport Management, College of Leisure and Recreation Management, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan
4Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
5Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
6Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
7Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
8National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 807, Taiwan
9Department of Family Medicine, Chi-Mei Medical Center, Tainan 710, Taiwan
10Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, 100 Shi-Chuan 1st Road, Kaohsiung 807, Taiwan

Received 22 March 2013; Accepted 9 May 2013

Academic Editor: Visith Thongboonkerd

Copyright © 2013 Ming-Hui Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Peritoneal dialysis (PD) is an increasingly accepted modality of renal replacement therapy. It provides the advantages of having a flexible lifestyle, stable hemodynamics, and better preservation of residual renal function. To enhance our understanding of the peritoneal dialysate of diabetes mellitus (DM), peritoneal dialysate proteins were identified by two-dimensional gel electrophoresis (2DE) combined with reverse-phase nano-ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (RP-nano-UPLC-ESI-MS/MS) followed by peptide fragmentation patterning. To validate the differential proteins, ELISA and Western blotting analyses were applied to detect candidate proteins that may be related to DM. We performed 2DE on the peritoneal dialysate samples, with detection of more than 300 spots. From this, 13 spots were excised, in-gel digested, and identified by RP-nano-UPLC-ESI-MS/MS. Ten of these showed significant differential expression between the DM and chronic glomerulonephritis (CGN) peritoneal dialysate samples. In this study, we conducted a comparative proteomic study on these two groups of dialysate that may provide evidence for understanding the different peritoneal protein changes. These proteins may not be new biomarkers; however, they may indicate a situation for possible drug treatment and can be the predictors of peritonitis for a validation study in the future.