Expression of N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for Pulmonary Metastasis
Figure 4
Effects of the knockdown of GalNAc4S-6ST on the invasion and migration of LLC cells. LLC cells stably expressing control-shRNA (filled bars) or GalNAc4S-6ST-shRNA (open bars) were plated on BD BioCoat chambers in the absence of fetal bovine serum. Cell invasion and migration were measured with or without Matrigel (BD Biosciences), respectively, as described in Section 2. Effects of the knockdown of GalNAc4ST-6ST on the invasion (a) and migration (b) of the LLC cells are summarized. The data represent the mean values ± S.D. for three clones of both control-shRNA (nos. 5, 10, and 14) and GalNAc4S-6ST-shRNA (nos. 7, 17, and 23). Three independent experiments were performed, and the representative results are shown. * versus control-shRNA by Student’s t-test.
