P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods
Intramethod technical replicability within ten independent biological samples. (a) Graphs show coefficient of variation (CV) of ten independent samples analyzed by flow cytometry after treatment with various concentrations of VRP (left) or PSC833 (right). A sample consists in the analysis of 10 000 cells. CV is expressed as the ratio of the standard deviation (SD) of the peak to the mean channel value (in percent) and provides a measure of the variability in signal intensity. (b) Coefficient of variation () of 6-plicated measurements of fluorescence obtained with a microplate reader for ten independent biological samples exposed to different concentrations of VRP.
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