Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin
Ability of CT-IC to detect the toxigenic V. cholerae strains. Fifteen ct gene-positive V. cholerae isolates and 5 ct gene-negative V. cholerae isolates were cultured under AKI-SW condition, and then obtained supernatants of each culture were examined by quantitative Bead-ELISA specific for CT (a) or CT-IC (b). For quantitative analysis, data are means ± SD of values from three independent experiments. UD: undetectable. For IC, the “+++”, “+”, or “−” symbols were placed on the left side of the strips developing “strong,” “faint,” or “no” bands at test lines, respectively. T: test line, C: control line.