Effects of Processing and Storage on Pediococcus pentosaceus SB83 in Vaginal Formulations: Lyophilized Powder and Tablets
Figure 3
Antimicrobial activity of Pediococcus pentosaceus SB83 against Listeria monocytogenes 2092 4b in simulated vaginal fluid at pH = 6.5. (a) Counts of viable cells of Listeria monocytogenes 2092 4b and (b) pH of simulated vaginal fluid during 24 h of contact, are represented. The tested conditions were SVF inoculated with bacterial suspension of P. pentosaceus SB83 and L. monocytogenes 2092 4b (◆); lyophilized of P. pentosaceus SB83 and L. monocytogenes 2092 4b (■); tablet of P. pentosaceus SB83 with retarding polymer and L. monocytogenes 2092 4b (▲), tablet of P. pentosaceus SB83 without retarding polymer and L. monocytogenes 2092 4b (). Controls were: SVF with 1 mg/mL of trypsin and inoculated with P. pentosaceus SB83 and L. monocytogenes 2092 4b (⋯), SVF inoculated with a non-bacteriocinogenic LAB strain ESB67 and L. monocytogenes 2092 4b (– –), and SVF inoculated with P. pentosaceus SB83 (—), SVF inoculated with L. monocytogenes 2092 4b (– ·). All points are means ± standard deviations.
