Research Article
Analysis of IL-1β Release from Cryopreserved Pooled Lymphocytes in Response to Lipopolysaccharide and Lipoteichoic Acid
Table 1
Experimental design for in vitro pyrogen testing.
| | Observation periods (days) |
Fresh blood (single donor) (hrs) |
Fresh lymphocytes (h) (single donor) |
Pooled lymphocytes cryopreserved in DMSO (h) |
Pooled lymphocytes cryopreserved in Glycerol (h) | | Pyrogens | | LPS (5 EU) | LTA (1 g/L) | LPS (5 EU) | LTA (1 g/L) | LPS (5 EU) | LTA (1 g/L) | LPS (5 EU) | LTA (1 g/L) |
| | 0 |
7 |
5 and 7 |
5 and 6 |
5 | 0 | 0 | 0 | 0 | | 7 | 5 | 3 and 4 | 3 | 4 | | 14 | 3 | 3 | 4 | 3 | | 30 | 3 | 3 | 3 | 4 | | 60 | 3 | 4 | 3 | 3 |
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