Figure 8: Twelve oocyte-like cells (OLCs) developed in vitro from putative ovarian stem cells (in two women with premature ovarian failure) cultured in a medium with added donated follicular fluid and analyzed by single-cell RT-PCR on the expression of genes related to pluripotency and oocytes. In OLC5 and OLC8 structures resembling germinal vesicles were observed; OLC8 expressed the meiosis-related gene SCP3. OLC12 developed a structure resembling zona pellucida (ZP) and indeed expressed the gene ZP3 (inverted microscope, Hoffman). Red Bar: 10 μm.