Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods

<table>Twelve oocyte-like cells (OLCs) developed <i>in vitro</i> from putative ovarian stem cells (in two women with premature ovarian failure) cultured in a medium with added donated follicular fluid and analyzed by single-cell RT-PCR on the expression of genes related to pluripotency and oocytes. In OLC5 and OLC8 structures resembling germinal vesicles were observed; OLC8 expressed the meiosis-related gene <i>SCP3</i>. OLC12 developed a structure resembling zona pellucida (ZP) and indeed expressed the gene <i>ZP3</i> (inverted microscope, Hoffman). <i>Red Bar</i>: 10 <i>μ</i>m.</table>

BioMed Research International

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Figure 8

Figure 8: Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods 