N2 Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
Figure 4
Effects of N2 gas plasma on nucleoprotein (NP) of influenza virus types A and B. Influenza A (a) and influenza B (b) viruses in nasal aspirate, influenza A virus (A/PR/8/34) in allantoic fluid (Viral titer: 3.16 1014 TCID50/mL) (c), and purified influenza viruses (d) were used. The samples were air-dried on cover glass and subjected to N2 gas plasma treatment (1.5 kpps) for the indicated time (min) using BLP-TES No.1. Samples were subsequently recovered in distilled water and then analyzed by immunochromatography (ESPRINE Influenza A & B-N, Fujirebio, Inc.). A and B indicate the lines for NP of influenza A virus and influenza B virus, respectively. The reference line (r) is also indicated. Influenza A virus H1N1 strain TW1: A/Taiwan/1/86 and H3N2 strain PM7: A/Panama/2007/99 were used. Influenza B virus TK53: B/Tokio/53/99 was also used.
