High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis

<table>Morphology of HDS and effects of culture media. Histology of the HDS stained for HE, row 1 and 2. HDS cultured in (a) Defined medium at 6 weeks, (b) Cardiac medium at 9 weeks (Defined medium for 6 weeks followed by Cardiac medium for 3 weeks), and (c) Endothelial medium at 4 weeks (Defined medium for 1 week followed by Endothelial medium for 3 weeks) are shown. Arrows are showing the additional multilayer area (b1) and buds (c1). Row 3 shows staining with antibody against Ki67 (green) detecting proliferating cells. DAPI (blue) was used to counterstain nuclei. The histogram is showing relative mRNA expression of the cardiac specific fibroblast marker <i>DDR2</i>.</table>

BioMed Research International

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Figure 2: High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis 

Figure 2 | High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis 