High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis
Figure 4
Endothelial phenotype in HDS. (a) Staining with antibody against VWF (yellow) shows randomly organized cells at 1 week in Defined medium. (b) At 6 weeks and (c) at 9 weeks the VWF positive cells were found mostly on the inside of cavities. DAPI (blue) was used to counterstain nuclei. (d) A vessel-like formation found in Endothelial medium at 4 weeks (Defined medium for 1 week followed by Endothelial medium for 3 weeks) in HE staining. (e) Millers elastin staining in human cardiac tissue (control) with blue staining for elastin in small vessels and (f) Millers elastin staining detecting another tubular structure in HDS cultured in Cardiac medium at 9 weeks (Defined medium for 6 weeks followed by Cardiac medium for 3 weeks). (g) Relative mRNA expression of endothelial-specific genes in HDS cultured in Defined media () for 1 week (early) and 4–6 weeks (late).
