Figure 4: Short-circuit current ( ) in permeabilized MDCK cells: Cl-free media. (a) A typical record is shown to demonstrate the experimental protocol. After equilibration (I), Cl was sequentially removed from the apical (II) and basal sides (III) of the monolayer. Then, amphotericin B (10 μM) was added to the apical medium, to permeabilize the apical plasma membrane. Next, the effects of ouabain (1 mM), furosemide (3 mM), and rotenone (10 μM) were evaluated. (b) Effect of ouabain (1 mM), furosemide (3 mM), and rotenone (10 μM) on of permeabilized MDCK cells. Results are mean ± SEM of five independent experiments.