Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs

Table 1

Kinetic parameters for substrates in 50 mM MOPS, 50 mM bicine, and 50 mM proline pH 9.0 at 30°C.

Substrate	𝐾𝑚; (mM)	𝑘cat; (s^−1)	𝑘cat/𝐾𝑚; (mM^−1s^−1)	Structure
L-cystathionine	1.07±0.22	92.67 ± 4.44	86.60 ± 21.46
L-djenkolic acid	0.23 ± 0.087	49.11 ± 3.08	213.50±94.14
Aminoethyl-L-cysteine	1.29±0.18	22.69 ± 0.75	17.59±3.03
L-cystine	1.14±0.22	4.67±0.25	4.10±1.01
L-cysteine	0.95±0.18	4.36±0.47	4.59±1.85
L-serine	8.28±1.57	2.65±0.13	0.32±0.07
O-acetyl-L-serine	11.21 ± 3.48	0.17 ± 0.018	0.015±0.006