Nonviral Methods for Inducing Pluripotency to Cells

Table 1

Advantages and disadvantages of vectors for iPS.

	Advantage	Disadvantage	Efficiency
Nonintegrating vector	Nonintegrating	Low efficiency, need for multiple rounds of transfection	0.001%
Episomal	Nonintegrating, single round of transfection	Low efficiency, labour intensive	0.001%
Minicircle	Nonintegrating, higher transfection efficiency	Potentially cytotoxic	0.005%