Research Article
Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design
Table 2
Experimental data values for particle size and entrapment of albumin nanoparticles obtained by Taguchi orthogonal array table of L9 Design.
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Particle size and surface charge (zeta potential) were determined in PBS at 25°C by dynamic light scattering using a NICOMP ZLS 380 analyzer (PSS-NICOMP, Santa Barbara, CA, USA) [8]. Entrapment efficiency was determined by employing Vivaspin 500 ultracentrifuge filters with molecular cut-off weight of 300 K Da (Viva Products, Inc., Littleton, MA, USA). Aqueous filtrate generated at the bottom of Vivaspin 500 ultracentrifuge tubes was subjected to UV spectrophotometric analysis to determine the concentration of GEM ( 248.6 nm; = 0.7053 × 104 L mol−1 cm−1) [13]. Results are presented as mean ± SD, . |