Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design

Table 2

Experimental data values for particle size and entrapment of albumin nanoparticles obtained by Taguchi orthogonal array table of L9 Design.

Albumin Concentration (% w/v)	Volume of BSA solution to total ethanol ratio (v/v)	Concentration diluted ethanol (% v/v)	Size (nm)	Entrapment efficiency (%)
3	1 : 0.75	100	20.01±1.17	61.06±1.94
3	1 : 0.90	70	15.72±0.83	76.87±2.03
3	1 : 1.05	40	24.69±0.69	69.95±3.84
4	1 : 0.75	70	20.14±0.86	72.38±2.42
4	1 : 0.90	40	25.65±1.71	70.98±1.06
4	1 : 1.05	100	88.90±3.08	78.64±1.97
5	1 : 0.75	40	58.17±1.95	61.75±0.84
5	1 : 0.90	100	191.53±14.96	86.97±2.71
5	1 : 1.05	70	130.69±5.27	83.01±2.09

Particle size and surface charge (zeta potential) were determined in PBS at 25°C by dynamic light scattering using a NICOMP ZLS 380 analyzer (PSS-NICOMP, Santa Barbara, CA, USA) [8]. Entrapment efficiency was determined by employing Vivaspin 500 ultracentrifuge filters with molecular cut-off weight of 300 K Da (Viva Products, Inc., Littleton, MA, USA). Aqueous filtrate generated at the bottom of Vivaspin 500 ultracentrifuge tubes was subjected to UV spectrophotometric analysis to determine the concentration of GEM (𝜆_max 248.6 nm; 𝜀₂₄₈ = 0.7053 × 10⁴ L mol⁻¹ cm⁻¹) [13].
Results are presented as mean ± SD, 𝑛=3.