Immune Monitoring in Cancer Vaccine Clinical Trials: Critical Issues of Functional Flow Cytometry-Based Assays

Figure 2

Comparison of different MHC multimers for detection of antigen-specific T-cells: dot plots representing percentages of CD8⁺ MART-1⁺ tetramer⁺/pentamer⁺/dextramers⁺ cells, analyzed within the singlets-live gate of a CD8⁺ expanded line obtained from a healthy donor.