Research Article

Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate

Table 1

Influence of pure carbohydrates and agroindustrial wastes on xylanase production by P. glabrum.

Carbon source 
(1% w/v)
Intracellular protein 
(mg)
Xylanase activity
(U/mL)(U/mg protein)

Pure carbohydrates

Glucose1.75 0.040.07
Xylose1.04 0.25 0.60
Maltose0.89 0.060.14
Lactose0.34 ND ND
Sucrose1.34 ND ND
Cellobiose1.11 ND ND
Avicel0.25NDND
CM-cellulose0.34NDND
Oat spelt xylan1.98 25.44 64.96

Agroindustrial wastes

Sugarcane bagasse0.538.3425.14
Wheat bran1.8420.54 43.35
Oat bran1.14 13.5428.31
Rice straw1.24 17.6530.34
Brewer's spent grain2.1034.32102.65
Soybean meal0.76 8.2320.43
Corn cobs0.83 7.3218.23
Citrus pectin0.34 0.100.34
Orange bagasse0.380.160.65